Institut Pasteur Course: Principles and applications of fluorescence microscopy – 10th Mar 2025 – 21st Mar 2025

Info, program and registration link: https://research.pasteur.fr/en/course/principles-and-applications-of-fluorescence-microscopy/

This course is designed for Master’s and international PhD students planning to use advanced live-cell fluorescence microscopy in their future research.

The use of fluorescence microscopy (wide field, confocal, multiphoton, and now superresolution) in combination with genetically encoded fluorescence probes comprise a powerful set of scientific tools to study live cells. However, surprising little practical and theoretical training in such methods exists within standard curricula, particular at the early stage of training (Masters or Doctorate level). This course offers to cover basic optics principles necessary to understand the origin of microscope resolution and design. Participants will get hands-on experience implementing simple optical configurations to illustrate these fundamental principles.  Subsequently, participants will perform experiments on state-of-the-art imaging equipment provided by microscope vendors.

Lectures will cover, in depth, the principle behind traditional high resolution imaging methods such as confocal, multi-photon, and the recently developed super-resolutions methods. Students will also learn about fundamental properties of synthetic and genetically encoded fluorescence indicators used for cellular morphology imaging and signaling recording. Finally guest lectures will demonstrate applications of some of the discussed methodologies.

Confirmed speakers / teachers:

• Kirsten BACIA (Martin Luther University Halle-Wittenberg, Germany)
• Robert CAMPBELL (University of Alberta, USA)
• Alexandra FRAGOLA (Université Paris Saclay France)
• Arnaud GAUTHIER (ENS Paris, France),
• Min-Hee KIM (Institut Pasteur, France)
• Anna-Maria LENNON (Institut Curie, France),
• Emailiano RONZITTI (Institut de la Vision, France)
• Audrey SALLES (Institut Pasteur, France)
• Kristine SCHAUER (Institut Gustave Roussy, France)
• Willy SUPATTO (École Polytechnique, France)
• Giuseppe VICIDOMINI (Istituto Italiano di Tecnologia Italy), Tim WAI (Institut Pasteur, France)